Inhibition by local anaesthetics of adenine nucleotide translocation in rat liver mitochondria

Adenine nucleotide translocation in liver mitochondria of hypothyroid rats.

In measurements using a disc filtration method, liver mitochondria obtained from hypothyroid rats translocate external ADP at 0°C via the atractyloside-sensitive carrier much more slowly than do mitochondria from normal rats, confirming the findings of Portnay et al. (Biochem. Biophys. I’t’es. Commun. 55, 17, 1973). The hypothyroid mitochondria contain 60% more ATP + ADP than do mitochondria fr...

The effect of butacaine on adenine nucleotide binding and translocation in rat liver mitochondria.

The effect of the local anaesthetic, butacaine, on adenine nucleotide binding and translocation in rat liver mitochondria partially depleted of their adenine nucleotide content was investigated. The range of butacaine concentrations that inhibit adenine nucleotide translocation and the extent of the inhibition are similar to the values obtained for native mitochondria. Butacaine does not alter ...

Kinetics and specificity of the adenine nucleotide translocation in rat liver mitochondria.

Inhibition by calcium of adenine nucleotide translocation in mitochondria isolated from Ehrlich ascites tumour cells.

A carrier-system specifically involved in transporting AdN has been shown to exist in mitochondria isolated from a variety of eukaryotic cells. This translocase which is inhibited by atractyloside is located in the inner membrane of these organelles and operates by catalyzing an exchange of endogenous with exogenous AdN; generally ADP is exchanged at a rate greater than that of ATP (for review ...

Acyl-CoA inhibition of adenine nucleotide translocation in ischemic myocardium.

The translocation of adenine nucleotides across the inner mitochondrial membrane and the tissue concentration of long-chain acyl-CoA esters were studied in dog heart after experimental myocardial ischemia. Ligation of the anterior coronary artery initiated events leading to an early decrease in adenine nucleotide translocase activity. A reciprocal increase in the concentration of heart tissue l...